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001003 opal 650 akoya bioscience  (Akoya Biosciences)


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    Structured Review

    Akoya Biosciences 001003 opal 650 akoya bioscience
    001003 Opal 650 Akoya Bioscience, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 1385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opal+650/Opal+650/pm41511877-283-101-104
    Average 99 stars, based on 1385 article reviews
    001003 opal 650 akoya bioscience - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Spatial Phenotype of the Mast Cell Population in Endometritis of Various Severities
    Article Snippet: Immune landscape , Tryptase + CD3 + CD8 + CD14 + CD68 + CD163 , OPAL 480, OPAL 540, OPAL 570, OPAL 620, OPAL 650, OPAL 690 , , DAPI (Akoya Biosciences, Marlborough, MA, USA).

    Article Title: Fat promotes growth and invasion in a 3D microfluidic tumor model of triple-negative breast cancer
    Article Snippet: Fluorescence in situ hybridization was performed using the standard RNAscope Multiplex Fluorescent V2 Assay (Advanced Cell Diagnostics) protocol for fixed-frozen samples.

    Multiplex Assay:

    Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation.
    Article Snippet: Discovery Inhibitor (Roche Tissue Diagnostics, 760-4840) was applied for 12 minutes to block any previous endogenous peroxidase. .. The primary- secondary- antibody pairs used in the multiplex panel alongside their assigned opal fluorophore were applied in a sequential manner, respectively, are as follows: pan-CKAE1-3 (Leica Biosciences, NCL-L-AE1-3, 1:250, 28 minutes), AR TI CL E IN P RE S ARTICLE IN PRESS OmniMap-anti Ms HRP (Roche Tissue Diagnostics, 760-4310, 12 minutes), Opal 620 (Akoya Biosciences, OP001004, 1:100, 8 minutes); Hypusine (Sigma-Aldrich, ABS1064-I, 1:100, 1 hour), Opal 520 (Akoya Biosciences, OP001001, 1:400, 8 minutes); Ki67 (30-9) (Roche Tissue Diagnostics, 790-4286, RTU, 20 minutes), OmniMap-antiRb HRP (Roche Tissue Diagnostics, 760-4311, 12 minutes), Opal 570 (Akoya Biosciences, OP001003, 1:200, 8 minutes); eIF5A (Abcam, ab137561,1:500, 32 minutes), OmniMap-antiRb HRP (Roche Tissue Diagnostics, 760- 4311, 12 minutes), Opal 690 (Akoya Biosciences, OP001006, 1:300, 8 minutes); SDHB (EPR10880), (Abcam, ab175225, 1:2000, 32 minutes), OmniMap-antiRb HRP (Roche Tissue Diagnostics, 760-4311, 12 minutes), Opal 650 (Akoya, OP001005, 1:300, 8 minutes). .. Following each detection with opal throughout the multiplex sequence, a de-crosslinking step was included to denature any previous antibody binding using a heat-and -pH mediated approach at 100oC using a pH 6 solution for 24 minutes (CC2, Roche Tissue Diagnostics, 950-123).

    Fluorescence:

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), https://doi.org/10.3390/cells15050432 Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Imaging:

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), https://doi.org/10.3390/cells15050432 Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Staining:

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Article Title: From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources
    Article Snippet: Subsequently, an HRP-conjugated secondary antibody (#MP-7452-15, ImmPRESS HRP, Vector Laboratories, Newark, NJ, USA) was applied. .. Visualization in fluorescence imaging was achieved using the TSA-Plus Cyanine 5 System (#NEL745001KT, Akoya Biosciences, Marlborough, MA, USA), DAPI (#H-1500, Nuclei staining, Vector Laboratories), and the Opal fluorophores Opal 480 (#FP150001KT), Opal 520 (#FP1487001KT), Opal 570 (#FP1488001KT), https://doi.org/10.3390/cells15050432 Opal 650 (#FP1496001KT), Opal 780 (#FP1501001KT) obtained from Akoya Biosciences (Marlborough, MA, USA). ..

    Amplification:

    Article Title: Spatiotemporal cellular map of the developing human reproductive tract.
    Article Snippet: .. Tyramide signal amplification with Opal 520, Opal 570 and Opal 650 (Akoya Biosciences) and TSA-biotin (TSA Plus Biotin kit, Perkin Elmer) and streptavidin-conjugated Atto 425 (Sigma Aldrich) was used to develop RNAscope probe channels. .. Stained sections were imaged with a Perkin Elmer Opera Phenix High-Content Screening system in confocal mode with 1-μm z step size, using a ×20 (NA 0.16, 0.299 μm pixel–1), ×40 (NA 1.1, 0.149 μm pixel–1) or ×63 (NA 1.15, 0.091 μm pixel–1) water-immersion objective.

    RNAscope:

    Article Title: Spatiotemporal cellular map of the developing human reproductive tract.
    Article Snippet: .. Tyramide signal amplification with Opal 520, Opal 570 and Opal 650 (Akoya Biosciences) and TSA-biotin (TSA Plus Biotin kit, Perkin Elmer) and streptavidin-conjugated Atto 425 (Sigma Aldrich) was used to develop RNAscope probe channels. .. Stained sections were imaged with a Perkin Elmer Opera Phenix High-Content Screening system in confocal mode with 1-μm z step size, using a ×20 (NA 0.16, 0.299 μm pixel–1), ×40 (NA 1.1, 0.149 μm pixel–1) or ×63 (NA 1.15, 0.091 μm pixel–1) water-immersion objective.



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